Figure 8
Expression of knock in target. Western blots show expression of recombinant human DNase I: Only transformed and heavy metal induced cells (+) show strong signals due to anti-DNase I antibodies. The intracellular PLA1-DNase-fusion protein is visualized on the left blot by samples of cell lysates. Bands are running at higher molecular weight than the mature and processed positive control due to the pre/pro-peptide. On the right, supernatants were subjected to Western blot; the size of the secreted protein of T. thermophila argues for a correct processing when compared to the positive control.