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Figure 4 | BMC Biotechnology

Figure 4

From: The bifunctional dihydrofolate reductase thymidylate synthase of Tetrahymena thermophila provides a tool for molecular and biotechnology applications

Figure 4

Generation of DHFR-TS deficient strains by allelic assortment. Wildtype strains are transfected with pKOI. In one copy of the 45 ARPs the endogenous DHFR-TS gene is substituted by the knock out construct (step 2). By amitotic division of the MAC and increasing selection pressure clones will arise, that have sorted out all endogenous DHFR-TS genes and retain only recombinant and defect DHFR-TS genes (step 3).

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