Figure 3From: The bifunctional dihydrofolate reductase thymidylate synthase of Tetrahymena thermophila provides a tool for molecular and biotechnology applicationspKOI: The basis for a DHFR-TS knock out construct. Figure 3 shows the knock out construct used for a targeted knock out of the DHFR-TS gene in T. thermophila. It consists of 3' and 5' flanking regions of the T. thermophila DHFR-TS gene and parts of its coding sequence (CDS), disrupted by a functional neomycin cassette conferring resistance to paromomycin. For more details refer to material and methods section.Back to article page