Table 2 Primers used to amplify the portions of S-layer protein gene of Lactobacillus crispatus (lbs), the green fluorescent protein gene (gfp mut2), and the erythromycin gene (ermAM) used as selective marker, respectively.

5'GGATCCCGGTCATTTTAACTTGCTA 3'

5'CTCGAGGATATCTGCAGCGTTAACAGAAACAGCTG 3'

lbs promoter plus leader peptide

344

5'GAATTCATGCACAACGCATTCTTCTATG 3'

5'AAGCTTCAGAAGATCCTATTAGAACTGTATGTTTAG 3'

lbs anchor plus terminator

600

5'CTCGAGGCTAGTAAAGGAGAAGAACTTTTCACT 3'

5'GAATTCTGCTTTGTATAGTTCATCCATGCCATG 3'

gfp mut2

720

5'GGGCCCTCTAGCACAAAAAGAAAAACG 3'

5'GGATCCTCTAGAGTCTAGGGACCTCTTTAGC 3'

erm AM

1200