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Table 1 Lactobacillus strains isolated from different portions of chicks gastrointestinal tract, species identification by PCR-ARDRA and some probiotic features

From: Genetic transformation of novel isolates of chicken Lactobacillusbearing probiotic features for expression of heterologous proteins: a tool to develop live oral vaccines

Isolate Species identificationa Bile salt inhibition (%)b Hydrophobicity (%)c H2O2 productiond
Gizzard    
1M14C L. reuteri 14 89 nde
1M14E L. johnsonii 59 35 +
2M14C L. reuteri 21 58 ++
2M14L L. acidophilus 58 52 +
2M14E L. acidophilus 92 88 +
3M14C L. reuteri 25 42 +
3M14L L. johnsonii 24 61 +
4M14C L. reuteri 19 64 +
4M14L L. reuteri 54 36 +
4M14E L. acidophilus 96 5 -
5M14C L. reuteri 18 78 +
5M14E L. vaginalis 100 75 ++
Small intestine    
1D14C L. salivarius 13 60 +++
2D14C L. vaginalis 71 67 ++
2D14E L. acidophilus 46 76 -
3D14C L. reuteri 13 83 ++
3D14L L. crispatus 36 88 ++
4D14C L. reuteri 13 88 +
4D14L L. reuteri 20 57 +
5D14E L. acidophilus 0 83 -
Large intestine    
1G14E Lactobacillus 27 78 -
2G14E L. acidophilus 37 55 -
3G14C L. reuteri 14 67 ++
3G14L L. johnsonii 22 45 -
4G14C L. vaginalis 37 25 ++
4G14L L. reuteri 40 35 ++
4G14E L. vaginalis 100 72 -
5G14C L. salivarius 43 66 +++
5G14L L. crispatus 41 35 +
Ceca    
2C14E L. acidophilus 72 55 -
2C14L L. acidophilus 10 78 -
3C14C L. reuteri 0 84 ++
3C14L L. johnsonii 24 83 +
3C14E L. acidophilus 80 64 +
4C14C L. salivarius 43 92 +++
4C14L L. acidophilus 73 79 nd
5C14C L. salivarius 41 83 +++
5C14E L. acidophilus 0 64 +
  1. aIsolates typed by 16S-23S rRNA PCR-ARDRA according to Moreira et al. (2005)
  2. bPercentage of optical density reduction after 6 h growing in MRS containing 0.3% bile salts
  3. cPercentage of microbial adhesion to xylene
  4. dH2O2 production in TMB-Plus (+++, dark blue; ++, blue; +, light blue/blue border; -, none)
  5. eNot determined