Genetic transformation of novel isolates of chicken Lactobacillusbearing probiotic features for expression of heterologous proteins: a tool to develop live oral vaccines

Table 1 Lactobacillus strains isolated from different portions of chicks gastrointestinal tract, species identification by PCR-ARDRA and some probiotic features

Isolate	Species identification^a	Bile salt inhibition (%)^b	Hydrophobicity (%)^c	H₂O₂ production^d
Gizzard
1M14C	L. reuteri	14	89	nd^e
1M14E	L. johnsonii	59	35	+
2M14C	L. reuteri	21	58	++
2M14L	L. acidophilus	58	52	+
2M14E	L. acidophilus	92	88	+
3M14C	L. reuteri	25	42	+
3M14L	L. johnsonii	24	61	+
4M14C	L. reuteri	19	64	+
4M14L	L. reuteri	54	36	+
4M14E	L. acidophilus	96	5	-
5M14C	L. reuteri	18	78	+
5M14E	L. vaginalis	100	75	++
Small intestine
1D14C	L. salivarius	13	60	+++
2D14C	L. vaginalis	71	67	++
2D14E	L. acidophilus	46	76	-
3D14C	L. reuteri	13	83	++
3D14L	L. crispatus	36	88	++
4D14C	L. reuteri	13	88	+
4D14L	L. reuteri	20	57	+
5D14E	L. acidophilus	0	83	-
Large intestine
1G14E	Lactobacillus	27	78	-
2G14E	L. acidophilus	37	55	-
3G14C	L. reuteri	14	67	++
3G14L	L. johnsonii	22	45	-
4G14C	L. vaginalis	37	25	++
4G14L	L. reuteri	40	35	++
4G14E	L. vaginalis	100	72	-
5G14C	L. salivarius	43	66	+++
5G14L	L. crispatus	41	35	+
Ceca
2C14E	L. acidophilus	72	55	-
2C14L	L. acidophilus	10	78	-
3C14C	L. reuteri	0	84	++
3C14L	L. johnsonii	24	83	+
3C14E	L. acidophilus	80	64	+
4C14C	L. salivarius	43	92	+++
4C14L	L. acidophilus	73	79	nd
5C14C	L. salivarius	41	83	+++
5C14E	L. acidophilus	0	64	+

^aIsolates typed by 16S-23S rRNA PCR-ARDRA according to Moreira et al. (2005)
^bPercentage of optical density reduction after 6 h growing in MRS containing 0.3% bile salts
^cPercentage of microbial adhesion to xylene
^dH₂O₂ production in TMB-Plus (+++, dark blue; ++, blue; +, light blue/blue border; -, none)
^eNot determined

ISSN: 1472-6750