Figure 3

Plasmid pLBS-GFP-Em^R constructed to transform chicken Lactobacillus. The promoter, leader peptide, C-terminal anchoring and terminator sequences of lbs gene of L. crispatus strain F5.7 were PCR amplified, cloned and assembled by overlapping restriction fragments onto pCR2.1-TOPO vector. The genes ermAM and gfp mut2 were also PCR amplified from other plasmids and used as selective marker and reporter gene, respectively.