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Figure 2 | BMC Biotechnology

Figure 2

From: A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects

Figure 2

Production of recombinant proteins. (A). Analysis of partially purified ButaIT by SDS-PAGE (Tris-Tricine buffer system); gel stained with Coomassie blue. Arrow indicates ButaIT polypeptide, based on reaction with anti-(his)6 antibodies on Western blot. (B), (C). Purification of ButaIT/GNA by gel filtration. Following partial purification by phenyl-Sepharose chromatography recombinant proteins were loaded onto a Sephacryl S200 column equilibrated in PBS buffer. (B) absorbance profile and (C) corresponding fractions run on SDS-PAGE (15 % acrylamide gel, stained with Coomassie blue). The arrow depicts purified ButaIT/GNA.

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BMC Biotechnology

ISSN: 1472-6750

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