Optical imaging of luminescence for in vivoquantification of gene electrotransfer in mouse muscle and knee

BMC Biotechnology

Table 2 Comparison of luminescence of tibial cranial muscles after electrotransfer of a luciferase-encoding plasmid and injection of luciferin i.p. (2.5 mg or 6 mg) or locally (0.1 mg)

Luciferin administration	*i.p.*		*local*
	*2.5 mg*	*6 mg*	*0.1 mg*
Luminescence 10⁵ units/min	2.05 ± 0.32	7.06 ± 2.79	18.42 ± 2.68

Values are mean ± SEM (n = 6 to 12) of the integrated values of luminescence in ROI of the tibial cranial muscle 3 days after electrotransfer of 3 μg of pC1 luc plasmid as measured in vivo after luciferin injection i.p. (2.5 mg/250 μl, n = 6 or 6 mg/250 μl, n = 6) or locally (0.1 mg/40 μl n = 12). Local injection of luciferin was performed 2 hours or more after the i.p. injection. Background luminescence was subtracted from the luminescence measured.

ISSN: 1472-6750