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Table 1 Comparison between local and i.p. injection of luciferin to induce luminescence after electrotransfer of a plasmid encoding luciferase into the knee joint or tibial cranial muscle

From: Optical imaging of luminescence for in vivoquantification of gene electrotransfer in mouse muscle and knee

  Experiment 1 (n = 5) Experiment 2 (n = 8)
  Knee 60 μg Muscle 3 μg Muscle 30 μg
  Local i.p. Local i.p. Local i.p.
Luminescence Millions units/min 4.37 ± 1.40 1.18 ± 0.25 3.08 ± 1.81 0.74 ± 0.38 20.41 ± 5.62 4.86 ± 1.20
Ratio local/i.p. 3.46 ± 0.76 3.55 ± 0.96 3.91 ± 0.38
Correlation r2 = 0.817 r2 = 0.929 r2 = 0.980
local/i.p. p = 0.0351 p = 0.0082 p < 0.0001
  1. Values are mean ± SEM of the integrated values of luminescence in ROI of the muscle or of the knee. In the first experiment, 60 and 3 μg of luciferase-encoding plasmid were electrotransferred into one knee joint and one tibial cranial muscle respectively. Luminescence was measured with the CCD camera 3 days after electrotransfer. In the second experiment, 30 μg of plasmid were electrotransferred into both tibial cranial muscles and luminescence was measured 7 days later. For all the experiments, luciferin was injected either locally (50 μg in the knee, 100 μg in the muscle) or i.p. (2.5 mg).