Feature | Position (bp) |
---|---|
Human CMV promoter | 1–589 |
Enhancer region | 59–465 |
AP1 binding site | 400–407 |
CAAT box | 521–525 |
TATA box | 554–560 |
Transcription start site | 583 |
Kozak translation initiation site | 606–612 |
pIN-G start codon | 613–615 |
Igκ-chain leader sequence | 613–675 |
Haemagglutinin epitope tag | 676–702 |
Unique Pvu I site | 708 |
Enhanced Green Fluorescent Protein (EGFP) | 742–1458 |
MCS-1 (BsrGI,AccIII,XhoI,Ecl136I*,SacI,HindIII,EcoRI,PstI,SalI) | 1451–1504 |
c-Myc epitope tag | 1504–1533 |
PDGFR transmembrane domain | 1537–1685 |
MCS-2 (KpnI, SacII,ApaI, SmaI*,BamHI,XbaI,BclI) | 1686–1718 |
pIN-G stop codon | 1723–1725 |
SV40 early mRNA polyadenylation signals | 1861–1866,1890–1895 |
mRNA ends | 1899,1911 |
F1 single-strand DNA origin | 1958–2413 |
Kanr bacterial promoter | 2475–2480,2498–2503 |
Kanr transcription start point | 2510 |
SV40 early promoter (tandem 72 bp) | 2587–2730 |
SV40 origin of replication | 2754–2889 |
Kanamycin/neomycin resistance gene | 2938–3732 |
Herpes simplex thymidine kinase poly-A signal | 3968–3973,3981–3986 |
pUC plasmid origin of replication | 4317–4960 |