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Table 1 Features of the pIN-G reporter system. Features of the pIN-G Trafficking Reporter Plasmid. Nucleotide positions of the corresponding features of pIN-G are indicated in the right hand column. Unique restriction sites for the extracellular and intracellular Multiple Cloning Sites (MCS-1 and MCS-2, respectively) are italicised. Asterisks denote enzymes yielding blunt ends.

From: PIN-G – A novel reporter for imaging and defining the effects of trafficking signals in membrane proteins

Feature Position (bp)
Human CMV promoter 1–589
Enhancer region 59–465
AP1 binding site 400–407
CAAT box 521–525
TATA box 554–560
Transcription start site 583
Kozak translation initiation site 606–612
pIN-G start codon 613–615
Igκ-chain leader sequence 613–675
Haemagglutinin epitope tag 676–702
Unique Pvu I site 708
Enhanced Green Fluorescent Protein (EGFP) 742–1458
MCS-1 (BsrGI,AccIII,XhoI,Ecl136I*,SacI,HindIII,EcoRI,PstI,SalI) 1451–1504
c-Myc epitope tag 1504–1533
PDGFR transmembrane domain 1537–1685
MCS-2 (KpnI, SacII,ApaI, SmaI*,BamHI,XbaI,BclI) 1686–1718
pIN-G stop codon 1723–1725
SV40 early mRNA polyadenylation signals 1861–1866,1890–1895
mRNA ends 1899,1911
F1 single-strand DNA origin 1958–2413
Kanr bacterial promoter 2475–2480,2498–2503
Kanr transcription start point 2510
SV40 early promoter (tandem 72 bp) 2587–2730
SV40 origin of replication 2754–2889
Kanamycin/neomycin resistance gene 2938–3732
Herpes simplex thymidine kinase poly-A signal 3968–3973,3981–3986
pUC plasmid origin of replication 4317–4960