Re-specification of the distribution of pIN-G by an autonomous targeting signal. HEK293 cells transiently transfected with pIN-G or pIN-TGN38 – a fusion construct corresponding to pIN-G bearing the autonomous targeting signal of the Trans-Golgi Network resident protein TGN-38 – were fixed, stained and imaged on a Delta-Vision workstation. Panel A, pIN-G; Panel B, pIN-TGN. HEK293 cells transiently transfected with pIN-TGN38, were fixed, permeabilised and treated with antibodies (See Methods) to the following organelle markers: C, GM130 (Golgi apparatus); D, EEA1 (early endosomes); E, Calnexin (endoplasmic reticulum) and F, lamp-1 (Lysosomes) followed by the appropriate Cy-3 conjugated secondary antibody. Green denotes pIN fluorescence while red corresponds to the organelle marker. Areas of red/green overlap are shown in yellow. Blue indicates DAPI-stained nuclei. Scale Bar: 15 μM.