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Table 2 acceptor vectors constructs

From: Modification of the Creator recombination system for proteomics applications – improved expression by addition of splice sites

Vector ID Vector Name Potential Splicing Events Tag Tag Position Promoter Function Expression Verification
V3 pLP-ECFP C1 (Clontech)* none ECFP N-terminus CMV localization Verified by Clontech
V4 pLP-EGFP C1 (Clontech) none EGFP N-terminus CMV localization Verified by Clontech
V5 pLP-EYFP C1 (Clontech) none EYFP N-terminus CMV localization Verified by Clontech
V6 pLPS-3'EGFP (Clontech) Splice for 3' Tags EGFP C-terminus CMV localization Verified by Clontech
V956 pLPS-3'ECFP Splice for 3' Tags ECFP C-terminus CMV localization Figure 3
V957 pLPS-3'ECFP-mito Splice for 3' Tags ECFP-mito C-terminus CMV targets to mitochondria Figure 3
V1662 pLP-RFP none RFP N-terminus CMV localization Additional File 5A
V1663 pLPS-3'RFP Splice for 3' Tags RFP C-terminus CMV localization Verified, Data not Shown
V1664 pLPS-3'RFP-mito Splice for 3' Tags RFP-mito C-terminus CMV targets to mitochondria Verified, Data not Shown
V25 pLP Flag none Flag N-terminus CMV IP/IB/IF Additional File 5C
V26 pLPS-3'Flag Splice for 3' Tags Flag C-terminus CMV IP/IB/IF Additional File 5B
V33 pLP Flag SD Splice for 5' Tags Flag N-terminus CMV IP/IB/IF Additional File 5C
V179 pLP Triple-Flag none Triple-Flag N-terminus CMV IP/IB/IF Figure 4&5
V180 pLP Triple-Flag SD Splice for 5' Tags Triple-Flag N-terminus CMV IP/IB/IF Figure 4&5
V181 pLPS 3' Triple-Flag Splice for 3' Tags Triple-Flag C-terminus CMV IP/IB/IF Figure 4&5
V207 pRETRO-Triple-Flag SD Splice for 5' Tags Triple-Flag N-terminus LTR IP/IB/IF/retrovirus% Additional File 5D
V143 pLP-HA SD Splice for 5' Tags HA N-terminus CMV IP/IB/IF Additional File 5E
V27 pLPS-3'HA Splice for 3' Tags HA C-terminus CMV IP/IB/IF Not expression verified
V516 pLP-dMyc none Double-Myc N-terminus CMV IP/IB/IF Figure 4
V517 pLP-dMyc SD Splice for 5' Tags Double-Myc N-terminus CMV IP/IB/IF Figure 4
V518 pLPS-3'dMyc Splice for 3' Tags Double-Myc C-terminus CMV IP/IB/IF Figure 4
V621 pGEX-4T-LP none GST N-terminus Tac Bacteria expression Verified, Data not Shown
V622 pGEX-2TK-LP none GST N-terminus Tac Bacteria expression Additional File 5F
V623 pAcGHLT-LP none GST His N-terminus Ph Baculovirus expression Not expression verified
V1579 pLP ProHis HTb none His N-terminus Trc Bacteria expression Additional File 5G
  1. *Abbreviations used: LP – loxP, mito – mitochondria localization peptide, SD – splice donor, IP – immunoprecipitation, IB – immunoblot analysis, IF – immunofluorescence, Ph – polyhedrin
  2. %V207 has been used extensively to produce protein from transient or stably transfected cells. It has not been tested for retrovirus production or resulting protein expression from a retrovirus.