Table 2 acceptor vectors constructs
Vector ID | Vector Name | Potential Splicing Events | Tag | Tag Position | Promoter | Function | Expression Verification |
|---|---|---|---|---|---|---|---|
V3 | pLP-ECFP C1 (Clontech)* | none | ECFP | N-terminus | CMV | localization | Verified by Clontech |
V4 | pLP-EGFP C1 (Clontech) | none | EGFP | N-terminus | CMV | localization | Verified by Clontech |
V5 | pLP-EYFP C1 (Clontech) | none | EYFP | N-terminus | CMV | localization | Verified by Clontech |
V6 | pLPS-3'EGFP (Clontech) | Splice for 3' Tags | EGFP | C-terminus | CMV | localization | Verified by Clontech |
V956 | pLPS-3'ECFP | Splice for 3' Tags | ECFP | C-terminus | CMV | localization | Figure 3 |
V957 | pLPS-3'ECFP-mito | Splice for 3' Tags | ECFP-mito | C-terminus | CMV | targets to mitochondria | Figure 3 |
V1662 | pLP-RFP | none | RFP | N-terminus | CMV | localization | |
V1663 | pLPS-3'RFP | Splice for 3' Tags | RFP | C-terminus | CMV | localization | Verified, Data not Shown |
V1664 | pLPS-3'RFP-mito | Splice for 3' Tags | RFP-mito | C-terminus | CMV | targets to mitochondria | Verified, Data not Shown |
V25 | pLP Flag | none | Flag | N-terminus | CMV | IP/IB/IF | |
V26 | pLPS-3'Flag | Splice for 3' Tags | Flag | C-terminus | CMV | IP/IB/IF | |
V33 | pLP Flag SD | Splice for 5' Tags | Flag | N-terminus | CMV | IP/IB/IF | |
V179 | pLP Triple-Flag | none | Triple-Flag | N-terminus | CMV | IP/IB/IF | Figure 4&5 |
V180 | pLP Triple-Flag SD | Splice for 5' Tags | Triple-Flag | N-terminus | CMV | IP/IB/IF | Figure 4&5 |
V181 | pLPS 3' Triple-Flag | Splice for 3' Tags | Triple-Flag | C-terminus | CMV | IP/IB/IF | Figure 4&5 |
V207 | pRETRO-Triple-Flag SD | Splice for 5' Tags | Triple-Flag | N-terminus | LTR | IP/IB/IF/retrovirus% | |
V143 | pLP-HA SD | Splice for 5' Tags | HA | N-terminus | CMV | IP/IB/IF | |
V27 | pLPS-3'HA | Splice for 3' Tags | HA | C-terminus | CMV | IP/IB/IF | Not expression verified |
V516 | pLP-dMyc | none | Double-Myc | N-terminus | CMV | IP/IB/IF | Figure 4 |
V517 | pLP-dMyc SD | Splice for 5' Tags | Double-Myc | N-terminus | CMV | IP/IB/IF | Figure 4 |
V518 | pLPS-3'dMyc | Splice for 3' Tags | Double-Myc | C-terminus | CMV | IP/IB/IF | Figure 4 |
V621 | pGEX-4T-LP | none | GST | N-terminus | Tac | Bacteria expression | Verified, Data not Shown |
V622 | pGEX-2TK-LP | none | GST | N-terminus | Tac | Bacteria expression | |
V623 | pAcGHLT-LP | none | GST His | N-terminus | Ph | Baculovirus expression | Not expression verified |
V1579 | pLP ProHis HTb | none | His | N-terminus | Trc | Bacteria expression |