Figure 3
Small scale expression, purification and cleavage of recombinant proteins. (A) SDS-PAGE analysis of the total cell lysate (T), insoluble (I), soluble (S), and the purified (P) fractions for each of the fusions: His-HHR23a, GST-UIM, MBP-α1-AT and Nus-UBL (the protein is marked with an asterisk). (B) SDS-PAGE analysis of each fusion during TEV protease digestion, (1) intact fusion (2) TEV digestion (3) purified protein after digestion. The band around 30 kDa observed in lane 2 of each experiment corresponds to the TEV protein.