ER-mediated luciferase activity after Ad-mediated transfer of shERα in vitro. (A) Schematic representation of the recombinant Ad vectors, carrying GFP and shERα expression cassettes that were used in this study. (B) EOMA and MXT cells were co-transfected with pERE-Luc and pCMV-LacZ and than infected either with Ad.Empty, Ad.shERα_1395, or Ad.shERα_1103. 10-9 M Estrogen was administrated for 24 hours. Luciferase activity was measured 48 hours after infection. Data represented as mean ± SD relative to infection with Ad.Empty.