Low density DNA microarray for detection of most frequent TP53 missense point mutations

BMC Biotechnology

Table 3 Detection of TP53 silent and missense base substitutions using DNA sequencing versus DNA microarray hybridization.

	DNA sequencing	Results of hybridization	Mutation
ID		Genotype
P1 Pp53	248 wtCGG→mutCGC	No wild type signal: mutant probe absent	Silent
	249 wtAGG→mutAGA	No wild type signal: p-249 G₂→T	Silent
	273 wtCGT	p-273 WT	Silent
P6 cDNA	248 wtCGG	p-248 WT	Silent
	249 wtAGG	p-249 WT	Silent
	273 wtCGT→mutCCT	No wild type signal: mutant probe absent	Proline
L1 A-427	248 wtCGG→mutCGT	p-248 WT: mutant probe absent	Silent
	249 wtAGG	p-249 WT	Silent
	273 wtCGT	p-273 WT	Silent
L2 C33 A	248 wtCGG	p-248 WT	Silent
	249 wtAGG	p-249 WT	Silent
	273 wtCGT→mutTGT	p-273 C→T	Cysteine
L4 Jurkat	248 wtCGG→mutCGK*	p-248 WT: mutant probe absent	Silent
	249 wtAGG→mutAGR**	p-249 G₂→T: mutant probe absent p-249 WT	Silent
	273 wtCGT	p-273 WT	Silent
L7 SW480	248 wtCGG	p-248 WT	Silent
	249 wtAGG→mutAGR**	p-249 G₂→T: mutant probe absent p-249 WT	Silent
	273 wtCGT→mutCAT	p-273 G→A	Histidine
L8 I-37	248 wtCGG→mutCGC	No wild type signal: mutant probe absent	Silent
	249 wtAGG→mutCGA	No wild type signal: mutant probe absent	Silent
	273 wtCGT→mutTGT	p-273 C→T	Cysteine
L9 I-51	248 wtCGG→mutCGC	No wild type signal: mutant probe absent	Silent
	249 wtAGG→mutCGA	No wild type signal: mutant probe absent	Silent
	273 wtCGT	p-273 WT	Silent
L10 PCS-4	248 wtCGG→mutCGC	No wild type signal: mutant probe absent	Silent
	249 wtAGG→mutCGA	No wild type signal: mutant probe absent	Silent
	273 wtCGT	p-273 WT	Silent
B3 Case 116	248 wtCGG	p-248 WT	Silent
	249 wtAGG	p-249 WT	Silent
	273 wtCGT→mutCTT	p-273 G→T	Leucine
B6 Case 152	248 wtCGG	p-248 WT	Silent
	249 wtAGG	p-249 WT	Silent
	273 wtCGT→mutCTT	p-273 G→T	Leucine

ISSN: 1472-6750