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Table 3 Detection of TP53 silent and missense base substitutions using DNA sequencing versus DNA microarray hybridization.

From: Low density DNA microarray for detection of most frequent TP53 missense point mutations

  DNA sequencing Results of hybridization Mutation
ID   Genotype  
P1 Pp53 248 wtCGG→mutCGC No wild type signal: mutant probe absent Silent
  249 wtAGG→mutAGA No wild type signal: p-249 G2→T Silent
  273 wtCGT p-273 WT Silent
P6 cDNA 248 wtCGG p-248 WT Silent
  249 wtAGG p-249 WT Silent
  273 wtCGT→mutCCT No wild type signal: mutant probe absent Proline
L1 A-427 248 wtCGG→mutCGT p-248 WT: mutant probe absent Silent
  249 wtAGG p-249 WT Silent
  273 wtCGT p-273 WT Silent
L2 C33 A 248 wtCGG p-248 WT Silent
  249 wtAGG p-249 WT Silent
  273 wtCGT→mutTGT p-273 C→T Cysteine
L4 Jurkat 248 wtCGG→mutCGK* p-248 WT: mutant probe absent Silent
  249 wtAGG→mutAGR** p-249 G2→T: mutant probe absent p-249 WT Silent
  273 wtCGT p-273 WT Silent
L7 SW480 248 wtCGG p-248 WT Silent
  249 wtAGG→mutAGR** p-249 G2→T: mutant probe absent p-249 WT Silent
  273 wtCGT→mutCAT p-273 G→A Histidine
L8 I-37 248 wtCGG→mutCGC No wild type signal: mutant probe absent Silent
  249 wtAGG→mutCGA No wild type signal: mutant probe absent Silent
  273 wtCGT→mutTGT p-273 C→T Cysteine
L9 I-51 248 wtCGG→mutCGC No wild type signal: mutant probe absent Silent
  249 wtAGG→mutCGA No wild type signal: mutant probe absent Silent
  273 wtCGT p-273 WT Silent
L10 PCS-4 248 wtCGG→mutCGC No wild type signal: mutant probe absent Silent
  249 wtAGG→mutCGA No wild type signal: mutant probe absent Silent
  273 wtCGT p-273 WT Silent
B3 Case 116 248 wtCGG p-248 WT Silent
  249 wtAGG p-249 WT Silent
  273 wtCGT→mutCTT p-273 G→T Leucine
B6 Case 152 248 wtCGG p-248 WT Silent
  249 wtAGG p-249 WT Silent
  273 wtCGT→mutCTT p-273 G→T Leucine
  1. *K = G or T (Heterozygous), **R = G or A (Heterozygous)