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Table 11 Comparing averaging across replicates versus not.

From: The case for well-conducted experiments to validate statistical protocols for 2D gels: different pre-processing = different lists of significant proteins

Averaged across replicates (54% of identified spots picked up) Log Normalized Log Transformed Missing replaced with – 17.28 (100% of identified spots picked up)
SSP 1134 SSP 1134
SSP 1509 SSP 1509
SSP 1733 SSP 1733
  SSP 2309
SSP 2864  
SSP 3222  
  SSP 3219
  SSP 3806
  SSP 4203
SSP 4225 SSP 4225
  SSP 4435
  SSP 4724
SSP 5413 SSP 5413
  SSP 6205
SSP 6236  
  SSP 6304
SSP 6314 SSP 6314
SSP 6349  
SSP 6452 SSP 6452
SSP 7144  
  SSP 7223
SSP 7439  
SSP 7750 SSP 7750
  1. Column 1 has the results of two sample t-tests when the intensity values were averaged across replicates. Columns 2 represents results of two sample t-tests when replicates were treated as independent observations.