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Table 1 The number of protein spots resolved in replicate gels of the same biological sample.

From: The case for well-conducted experiments to validate statistical protocols for 2D gels: different pre-processing = different lists of significant proteins

Sample No (Treatment group) No. of Replicates Total Proteins resolved (occurring in at least one replicate) (%) Number resolved in all replicates (%) Number resolved in all but one replicate (%) Number resolved in all but two (%) Number resolved in just one (%)
6 (GSE) 3 4064 (100) 309 (8) 563 (14) 3192 (78) -
7 (GSE) 4 546 (100) 169 (31) 130 (26) 97 (18) 150 (27)
8 (GSE) 4 954 (100) 186 (19) 120 (12) 105 (11) 543 (57)
9 (GSE) 2 904 (100) 342 (39) 562 (62) - -
10 (GSE) 2 396 (100) 229 (58) 167 (42) - -
22 (CONT) 4 924 (100) 234 (25) 89 (10) 109 (12) 492 (53)
23 (CONT) 4 950 (100) 161 (17) 151 (16) 102 (11) 536 (59)
24 (CONT) 2 879 (100) 312 (35) 567 (65) - -
25 (CONT) 3 957 (100) 272 (28) 117 (12) 568 (59)  
26 (CONT) 2 432 (100) 183 (42) 249 (58) - -
  1. The variability in the resolution of protein spots in technical replicates in a 2D gel experiments is one of the causes of the large number of missing spot intensities. The variability in the table above demonstrates the need for technical replicates as a quality control measure to identify spots that are most 'reliable' and common and therefore most useful to generalize to a larger population.