Skip to main content

Table 2 Silencing effciencies after transfection of various dsRNAs.

From: Functional complementation of RNA interference mutants in trypanosomes

Cell line

TbPFR2 silencing in the new flagellum (% bi-flagellated cells)

 

dsRNA transfected

 

TbPFR2 CDS

TbPFR2 UTRs MIX

WT

88 % (n = 53)

62 % (n = 147)

TbPFR2tag

68 % (n = 113)

2 % (n = 51)

TbPFR2tag-ΔHLA

78 % (n = 100)

72 % (n = 100)

  1. WT, TbPFR2tag and TbPFR2tag-ΔHLA trypanosomes were transfected with GFP, TbPFR2 CDS or TbPFR2 UTRs MIX dsRNAs and bi-nucleated/bi-flagellated cells were counted for the status of their new flagellum. The data presented in this table correspond to the immunofluorescence experiment described in Figure 3 (see text for details).
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us