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Table 2 Silencing effciencies after transfection of various dsRNAs.

From: Functional complementation of RNA interference mutants in trypanosomes

Cell line TbPFR2 silencing in the new flagellum (% bi-flagellated cells)
  dsRNA transfected
  TbPFR2 CDS TbPFR2 UTRs MIX
WT 88 % (n = 53) 62 % (n = 147)
TbPFR2tag 68 % (n = 113) 2 % (n = 51)
TbPFR2tag-ΔHLA 78 % (n = 100) 72 % (n = 100)
  1. WT, TbPFR2tag and TbPFR2tag-ΔHLA trypanosomes were transfected with GFP, TbPFR2 CDS or TbPFR2 UTRs MIX dsRNAs and bi-nucleated/bi-flagellated cells were counted for the status of their new flagellum. The data presented in this table correspond to the immunofluorescence experiment described in Figure 3 (see text for details).