Table 1 Silencing effciencies after transfection of various dsRNAs. WT trypanosomes were transfected with TbPFR2, FLA1 or GFP dsRNA.
From: Functional complementation of RNA interference mutants in trypanosomes
| Â | Â | Time (hours) | ||
|---|---|---|---|---|
dsRNA | Phenotype (%) | 0 h | 15 h | 22 h |
TbPFR2 | TbPFR2 silencing | 0 | 61.2 (n = 1069) | 74.2 (n = 1069) |
FLA1 | FLA1 silencing | 0 | 56 (n = 586) | 54 (n = 472) |
TbPFR2 + GFP | TbPFR2 silencing | 0 | 56.2 (n = 301) | 70.9 (n = 243) |
TbPFR2 + FLA1 | TbPFR2 silencing | 0 | 53.6 (n = 349) | 69.7 (n = 210) |
| Â | FLA1 silencing | 0 | 59 (n = 349) | 53 (n = 210) |