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Figure 1 | BMC Biotechnology

Figure 1

From: Assembly of a gene sequence tag microarray by reversible biotin-streptavidin capture for transcript analysis of Arabidopsis thaliana

Figure 1

Solid-phase purification parameters. In A the agarose gel image shows the first (upper left image) and second (bottom left image) elution of the captured 1.0 kb product. The first and fourth lanes contain a size marker, while the second and third lanes contain an equal amount, assuming 100% yield at purification step, of unpurified PCR product (PP) and purified PCR product (eluate), respectively. The images on the right shows the results from the carry-over test, where PCR product and water were used as input samples for multiple consecutive purification reactions in an alternating order. The upper part shows the hybridisation results (PCR product, water, PCR product and water), while the box-and-whiskers plot below shows the quantifications of the signals (n = 18, six replicates of the three different products). Purification of three amplification products (red line 0.5 kb, green line 1.0 kb and blue line 1.3 kb) is investigated using an increasing amount of streptavidin-coated magnetic beads (number of independent replications, n = 8, panel B), varying binding time (n = 4, panel C) and repeatedly used beads (n = 4, panel D). The black line in (D) is based on fluorescence data and is plotted using the y-axis on the right, while the three other lines are based on absorbance measurements and use the y-axis on the left side. The presented data originates from repeated independent experiments and the error bars denote the calculated standard error.

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