Table 1 Deduced amino acid sequence of the heavy and light chain variable regions of human scFv and diabody.
scFv | FR1 | CDR1 | FR2 | CDR2 |
|---|---|---|---|---|
MuVHGuy13 | QVKLQESGPDLVKPGASVKISCKASGYTFT | DYNIH | WVKQSRGKSLEWIG | YIYPYNGNTYYNQKFKN |
MuVLGuy13 | DIELTQSPAIMSASPGEKVTITC | SASSSVSYMH | WFQQKPGTSPKLWLY | STSNLAS |
HuVHB10 | QVQLQESGAEVKKPGLGEGLLQASGGTFS | RYALS | WVRQAPGQGLEWMG | GIIPIFGTTNYAQKFQG |
HuVLB10 | DIQMTQSPSSLSASVGDRVTITC | RASQGISNYLA | WFQQKPGKAPKSLIY | AASSLQS |
HuVHD12 | QVQLQEXGAEVKKPGESLKIXCKGSGYSFT | SYWIG | WVRQMPGKGLEWMG | IIYPGDXDTRYSPSFQG |
HuVLD12 | DIQMTQSPSTLSASIGDRVTITC | RASEGIYHWLA | WYQQKPGKAPKLLIY | EASRLQS |
HuVHH6 | QVQLQESGGGVVQPGRSLRLSCAASGFTFS | SYAMH | WVRQAPGKGLEWVS | YISSSGSYIYYADSVKG |
HuVLH6 | DIVMTQSPSSLSASVGDRVTITC | RASQGISNYLA | WFQQKPGKAPKSLIY | AASSLQS |
scFv | FR3 | CDR3 | FR4 | Â |
MuVHGuy13 | KATLTVDNSSTSAYMELRSLTSEDSAVYYCAT | YFDY | WGQGTTVTVS | Â |
MuVLGuy13 | GVPARFSGSGSGTSYSLTISRMEAEDAATYYC | HQRTSYPYT | FGGTKLEIKR | Â |
HuVHB10 | RVTIAADESTSTAYLELSSLRSEDTALYYCAK | SYDYVWGSYRPNEYGLDI | WGQGTMVTVS | Â |
HuVLB10 | GVPSKFSGSGSGTEFTLTISSLQPEDFATYYC | QELISYPLT | FGGGTKLEIKRA | Â |
HuVHD12 | QVTISADKSISTAYLQWSSLKASDTAMYYCAR | LGLQDDYVWGSXNWFDP | WGQGTLVTVSTS | Â |
HuVLD12 | GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC | LQDFTYPRT | FGQGTKVEIKRA | Â |
HuVHH6 | RFTISRDNAKNSLYLQMNRLRAEDTAVYYCAR | DMAGTSYYYYYMDV | WGKGTLVTVSTS | Â |
HuVLH6 | GVPSKFSGSXSGTEFTLTISSLQPEDFATYYC | QELISYPLT | FGGGTKLEIKRA | Â |