Figure 3

Inhibition of chimeric scFv (mGuy13VH/huVL) to SAI/II by the monoclonal Ab Guy13. Chimeric scFv were allowed to bind to SAI/II followed by replacement with an access of mAb Guy 13. The experiments were carried out in duplicate and the mean values are shown. Bound scFv were detected via their MYC-tag. The murine mAb Guy13 bound to the SAI/II. The scFv Guy13 was not replaced by an unrelated mAb (ctrl.) Binding of A1 and G4 was only inhibited by 36% and 25%, respectively indicating that these scFv might recognize a different epitope than mAb Guy13. The binding of chimeric scFv A6, A9, B4, and C6 were inhibited between 77–84% indicating that they recognize the same epitope as mAb Guy13.