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Figure 3 | BMC Biotechnology

Figure 3

From: Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells

Figure 3

Intra- and extracellular concentrations of recombinant rhVEGF. Physcomitrella protoplasts were transiently transfected with pVSP-VEGF and pASP-VEGF in four (pVSP-VEGF and control) and seven (pASP-VEGF) independent transfections. After 5 days the concentrations of secreted and intracellularly retained recombinant protein were measured by ELISA. A: mean values of concentrations of extra- and intracellular rhVEGF measured by ELISA. B: Western blot with culture medium of transiently rhVEGF-producing cell. Control: untransfected moss protoplasts.

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