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Figure 2 | BMC Biotechnology

Figure 2

From: Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells

Figure 2

Concentrations of secreted recombinant GST. Physcomitrella protoplasts were transiently transfected with pVSP-GST and pASP-GST in eight independent transfections. After 5 days the concentrations of secreted and intracellularly-retained recombinant protein were measured by ELISA. Additionally, GST was affinity-purified from both medium and supernatant and detected by western blot. A: Culture medium of two transfections was pooled. Mean values of eight transfections are given. B: Protoplasts of eight transfections were pooled and GST was affinity-purified from the crude extracts. Values reflect the means of the two measurements. Control: mock-transfected protoplasts.

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