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Figure 6 | BMC Biotechnology

Figure 6

From: Selection and characterization of a promoter for expression of single-copy recombinant genes in Gram-positive bacteria

Figure 6

Western blot analysis of recombinant S. gordonii and B. subtilis strains expressing M6 protein and M6/TTFC fusion protein. (A) S. gordonii envelope fractions. Lane 1 through 3, GP1241 expressing M6 under the control of PP promoter. Lane 1, GP1241 harvested after overnight growth. Lane 2, GP1241 harvested after early stationary phase. Lane 3, GP1241 harvested after exponential phase. Lane 4, recipient strain GP201 (negative control). Lane 5, GP231 (positive control). Blot was developed with anti-M6 monoclonal antibody 10B6. (B) S. gordonii and B. subtilis envelope fractions. Lane 1, S. gordonii GP204 (negative control). Lane 2, S. gordonii GP1253 expressing M6/TTFC (positive control). Lane 3, B. subtilis recipient strain GP800.2 (negative control). Lane 4, B. subtilis GP848 expressing M6/TTFC under the control of PP promoter. Blot was developed with anti TTFC rabbit serum. Molecular weight markers are shown in the right side of panels.

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BMC Biotechnology

ISSN: 1472-6750

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