Figure 2

Schematic representation of the S. gordonii locus containing PP promoter. DltD orthologue, an ORF encoding a putative acetyltransferase (ORF2), ORF1, the gene encoding a Tmp7 transmembrane protein, and the cat gene are indicated by arrows. Dashed arrows designate that the ORF is only partially represented in the scheme. The 600 bp TaqI-HindIII fragment including PP promoter and cloned upstream of the cat gene in pVMB5 is indicated by a dotted box. Nucleotide sequence of the PP promoter region (332 bp) is reported inside the box. The transcriptional start site determined by primer extention analysis is marked with an asterisk. Proposed -35 and -10 regions are underlined. A second putative -10 sequence is overlined. ORF1 putative ribosome binding site (RBS) is boxed. ATG initiation codon of cat is in bold characters and the sequence of HindIII site is in italic letters. The complete sequence of the 600 bp cloned fragment is available on GenBank (Accession number: U74080). T, TaqI site; B, BamHI site; H, HindIII site ; loop, putative transcriptional terminator.