Effect of electroporation on GFP expression in zebrafish. Fluorescence images from control (A) and electroporated samples (B, C and D). (A) is an image taken from the tissue at the site of injection. B, C and D represent the images obtained from head, mid-region and tail respectively. Fluorescence from tissue sections from head and tail of control fish is similar to (A), hence not shown. At least 20 fish were electroporated in different days with pCMVGFP. We observe some variation in extent of GFP expression but the expression was always maximum in the mid-region. The presented data is from one fish.