Influence of various parameters of electroporation on reporter gene activities in adult zebrafish and Indian Carp. Electrical pulses were applied following intra-muscular injection of plasmid DNA: A, Pulse number; B, Voltage strength; C, Amount of plasmid DNA present in fixed volume with (open circles) and without (closed circles) electroporation. D, Effect of electroporation (40 V·cm-1 and 6 pulses) on 2–3 month old Indian carp. Controls received only the plasmid in injection. Relative luminescence units were normalized for the amount of protein. Each data point is an average of values obtained from 6–8 fish and each experiment was repeated three times.