Table 2 Structural properties of avidin and avidin related proteins. Elution times from FPLC gel filtration and calculated molecular weights of the proteins. Heat-induced unfolding temperatures of proteins from DSC analysis (average ± S.D).
| Â | Gel filtration | DSC | |||
|---|---|---|---|---|---|
Protein | Elution time (min) | Molecular mass (kDa) | Tm (-biotin) (°C) | Tm (+biotin) (°C) | ΔTma (°C) |
AVD b | 31.5 | 46.5 | 83.5 ± 0.1 | 117.0 ± 0.7 | 33.5 |
AVD(K111I) | 31.9 | 42.1 | 76.5 ± 0.1 | 116.0 ± 0.1 | 39.5 |
AVR2 | 30.1 | 66.6 | 91.3 | 112.5 | 21.2 |
AVR2(I109K) | 30.0 | 68.2 | 97.6 ± 0.1 | 118.7 ± 0.1 | 21.1 |
AVR2-b | 30.9 | 55.1 | 93.4 ± 0.4 | 112.5 | 19.1 |
AVR4 c | 31.9 | 42.1 | 106.4 ± 0.8 | 125.4 ± 0.8 | 19.0 |
AVR4(K109I) | 31.3 | 49.8 | 104.5 ± 0.5 | 118.6 ± 1.2 | 14.1 |
AVR6-b | 31.1 | 51.5 | 87.7 | 114.0 | 26.3 |