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Table 2 Structural properties of avidin and avidin related proteins. Elution times from FPLC gel filtration and calculated molecular weights of the proteins. Heat-induced unfolding temperatures of proteins from DSC analysis (average ± S.D).

From: Avidin related protein 2 shows unique structural and functional features among the avidin protein family

  Gel filtration DSC
Protein Elution time (min) Molecular mass (kDa) Tm (-biotin) (°C) Tm (+biotin) (°C) ΔTma (°C)
AVD b 31.5 46.5 83.5 ± 0.1 117.0 ± 0.7 33.5
AVD(K111I) 31.9 42.1 76.5 ± 0.1 116.0 ± 0.1 39.5
AVR2 30.1 66.6 91.3 112.5 21.2
AVR2(I109K) 30.0 68.2 97.6 ± 0.1 118.7 ± 0.1 21.1
AVR2-b 30.9 55.1 93.4 ± 0.4 112.5 19.1
AVR4 c 31.9 42.1 106.4 ± 0.8 125.4 ± 0.8 19.0
AVR4(K109I) 31.3 49.8 104.5 ± 0.5 118.6 ± 1.2 14.1
AVR6-b 31.1 51.5 87.7 114.0 26.3
  1. aΔTm is the change in Tm upon addition of a three-fold molar excess of D-biotin.
  2. bDSC results for avidin are from Ref [61].
  3. cDSC results for AVR4 are from Ref [36].