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Figure 1 | BMC Biotechnology

Figure 1

From: Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy

Figure 1

Inhibition of PrP aggregation in serum. FITC-labeled recombinant bovine prion protein (concentration 10 nM) was incubated at 37°C for 20 h with continuous shaking, either in 150 μl PBS (left panel) or in the same volume of serum (right panel), followed by flow cytometry. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Fluorescence 2 (FL2-H) dot-plot. The number of counts in the area containing specific signals (R2) is given in the figures. Aggregate formation in serum is strongly inhibited.

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