Time course of eye coloration change in Sa_v knockdown grasshopper nymphs. Anterolateral views of wild type (a-e) and Sa_v knockdown (f-j) grasshopper eyes at different stages of development. Anterior is to the right and dorsal up. Numbers denote instar during which the corresponding vertical eye stripe has been formed. (a,f) second instar. (b,g) third instar. (c,h) fourth instar. (d,i) fifth and final nymphal instar. (e,j) adult eye. Note bright red brown coloration of stripes one and two in panels g and h compared to dark brown coloration in the embryonic cap (ec), the pigmented compound eye area formed in the embryo. Also in the adult eye of the Sa_v knockdown animal shown in panel j, stripes one and two are more lightly coloured than the later, more anterior stripes.