From: Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance
gDNA Input (ng) | Completed Genotypes1 | % Completion | Undeter. Genotypes | % Undeter. | No Amp. | % No Amp. | Concordant Genotypes | % Concordance | Discordant Genotypes | % Discordance |
---|---|---|---|---|---|---|---|---|---|---|
1 ng gDNA or wgaDNA input into TaqMan® SNP genotype assay2,3 | ||||||||||
gDNA | 2636 | 99.62 | 10 | 0.38 | 0 | 0 | 2636 | 100.00 | 0 | 0.00 |
1 | 25524 | 96.45 | 945 | 3.55 | 0 | 0 | 2546 | 99.76 | 68 | 0.24 |
10 | 2627 | 99.28 | 19 | 0.72 | 0 | 0 | 2627 | 100.00 | 0 | 0.00 |
25 | 2636 | 99.62 | 10 | 0.38 | 0 | 0 | 2635 | 99.96 | 1 | 0.04 |
50 | 2614 | 98.79 | 316 | 1.17 | 1 | 0.04 | 2614 | 100.00 | 0 | 0.00 |
100 | 2620 | 99.02 | 256 | 0.94 | 1 | 0.04 | 2620 | 100.00 | 0 | 0.00 |
200 | 2623 | 98.07 | 21 | 0.79 | 2 | 0.08 | 2622 | 99.96 | 1 | 0.04 |
4 ng gDNA or wgaDNA into TaqMan ® SNP genotype assay 4 | ||||||||||
gDNA | 2634 | 99.55 | 12 | 0.45 | 0 | 0.00 | 2634 | 100.00 | 0 | 0.00 |
1 | 25894 | 97.85 | 555 | 2.08 | 2 | 0.08 | 2586 | 99.88 | 3 | 0.12 |
10 | 2628 | 99.32 | 17 | 0.64 | 1 | 0.04 | 2627 | 99.96 | 1 | 0.04 |
25 | 2629 | 99.36 | 14 | 0.53 | 3 | 0.11 | 2629 | 100.00 | 0 | 0.00 |
50 | 2621 | 99.06 | 235 | 0.87 | 2 | 0.08 | 2619 | 99.92 | 2 | 0.08 |
100 | 2619 | 98.98 | 205 | 0.76 | 77 | 0.26 | 2617 | 99.92 | 2 | 0.08 |
200 | 2635 | 99.4 | 10 | 0.38 | 1 | 0.04 | 2632 | 99.89 | 3 | 0.11 |
20 ng gDNA or wgaDNA into TaqMan ® SNP genotype assay | ||||||||||
gDNA | 2637 | 99.66 | 9 | 0.34 | 0 | 0 | 2637 | 100.00 | 0 | 0.00 |
1 | 25694 | 97.09 | 775 | 2.91 | 0 | 0 | 2566 | 99.88 | 3 | 0.12 |
10 | 2633 | 99.51 | 13 | 0.49 | 0 | 0 | 2633 | 100.00 | 0 | 0.00 |
25 | 2639 | 99.74 | 7 | 0.26 | 0 | 0 | 2638 | 99.96 | 1 | 0.04 |
50 | 2633 | 99.51 | 13 | 0.49 | 0 | 0 | 2632 | 99.96 | 1 | 0.04 |
100 | 2630 | 99.40 | 16 | 0.60 | 0 | 0 | 2629 | 99.96 | 1 | 0.04 |
200 | 2635 | 99.55 | 11 | 0.42 | 0 | 0 | 2632 | 99.89 | 3 | 0.11 |