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Table 3 Genotype Quality (GQ) scores of concordant STR genotypes

From: Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance

gDNA Input (ng) GQ1Category, Heterozygotes
  I II III Total
gDNA 5 53 580 6382
1 43 137 256 4363
10 27 140 451 618
25 26 126 463 615
50 21 130 474 625
100 25 110 482 617
200 78 31 537 646
Total 225 727 3243 4195
gDNA Input (ng) GQ Category, Homozygotes
  I II III Total
gDNA 0 9 204 2134
1 9 25 168 2025
10 2 8 202 212
25 1 8 199 208
50 4 11 196 211
100 8 10 191 209
200 8 20 184 212
Total 32 91 1344 1467
  1. 1Category I = GQ scores, originally ≥ 0.25 and <0.40, that have been successfully edited; Category II = GQ scores ≥ 0.40 and ≤ 0.50; Category III = GQ scores ≥ 0.79 and ≤ 0.90. 2GQ score distribution, heterozygote genotypes, gDNA versus all wgaDNA, p < 0.0001. 3GQ score distribution, heterozygote genotypes, wgaDNA produced from 1 ng gDNA input versus other gDNA inputs, p < 0.0001.4GQ score distribution, homozygote genotypes, gDNA versus wgaDNA produced from 1, 100, 200 ng gDNA input, p < 0.0001, 0.01, 0.0001, respectively. 5GQ score distribution, homozygote genotypes, wgaDNA produced from 1 ng gDNA input versus 10, 25, 50, 100 ng gDNA inputs, p = 0.0003, 0.0001, 0.009, 0.02, respectively.
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