gDNA Input (ng)
|
GQ1Category, Heterozygotes
|
---|
|
I
|
II
|
III
|
Total
|
---|
gDNA
|
5
|
53
|
580
|
6382
|
1
|
43
|
137
|
256
|
4363
|
10
|
27
|
140
|
451
|
618
|
25
|
26
|
126
|
463
|
615
|
50
|
21
|
130
|
474
|
625
|
100
|
25
|
110
|
482
|
617
|
200
|
78
|
31
|
537
|
646
|
Total
|
225
|
727
|
3243
|
4195
|
gDNA Input (ng)
|
GQ Category, Homozygotes
|
|
I
|
II
|
III
|
Total
|
gDNA
|
0
|
9
|
204
|
2134
|
1
|
9
|
25
|
168
|
2025
|
10
|
2
|
8
|
202
|
212
|
25
|
1
|
8
|
199
|
208
|
50
|
4
|
11
|
196
|
211
|
100
|
8
|
10
|
191
|
209
|
200
|
8
|
20
|
184
|
212
|
Total
|
32
|
91
|
1344
|
1467
|
- 1Category I = GQ scores, originally ≥ 0.25 and <0.40, that have been successfully edited; Category II = GQ scores ≥ 0.40 and ≤ 0.50; Category III = GQ scores ≥ 0.79 and ≤ 0.90. 2GQ score distribution, heterozygote genotypes, gDNA versus all wgaDNA, p < 0.0001. 3GQ score distribution, heterozygote genotypes, wgaDNA produced from 1 ng gDNA input versus other gDNA inputs, p < 0.0001.4GQ score distribution, homozygote genotypes, gDNA versus wgaDNA produced from 1, 100, 200 ng gDNA input, p < 0.0001, 0.01, 0.0001, respectively. 5GQ score distribution, homozygote genotypes, wgaDNA produced from 1 ng gDNA input versus 10, 25, 50, 100 ng gDNA inputs, p = 0.0003, 0.0001, 0.009, 0.02, respectively.