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Table 1 STR genotyping performance

From: Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance

gDNA Input (ng) Completed1 % Completion No Amplification GQ<0.252 Concordant % Concordance Discordant Genotypes % Discordance
gDNA 864 100.03 0 13 851 100.04 0 0.0
1 821 95.0 43 70 638 80.1 150 19.0
10 862 99.8 2 9 830 97.1 25 2.9
25 863 99.9 1 22 823 97.7 19 2.3
50 863 99.9 1 15 836 98.6 12 1.4
100 861 99.7 3 11 826 96.8 27 3.2
200 864 100.0 0 0 858 99.3 6 0.7
  1. 1N = 27 gDNA or wgaDNA samples were genotyped in duplicate using the AmpFlSTR® Identifiler® assay for N = 864 attempted genotypes/sample. 2A Genotype Quality Score (GQ) of <0.25 indicates a STR genotype with a Genotype Quality Score beloew the calling threshold of GeneMapper v3.0 software. 3gDNA exhibited significantly greater STR genotype completion rate than did 1 ng gDNA input (p < 0.001). 4gDNA exhibited significantly greater STR genotype concordance rates compared to wgaDNA (p < 0.0001, except for 50 ng gDNA input, with p = 0.001, and 200 ng gDNA input, p = 0.03).
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