Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance

BMC Biotechnology

Table 1 STR genotyping performance

gDNA Input (ng)	Completed¹	% Completion	No Amplification	GQ<0.25²	Concordant	% Concordance	Discordant Genotypes	% Discordance
gDNA	864	100.0³	0	13	851	100.0⁴	0	0.0
1	821	95.0	43	70	638	80.1	150	19.0
10	862	99.8	2	9	830	97.1	25	2.9
25	863	99.9	1	22	823	97.7	19	2.3
50	863	99.9	1	15	836	98.6	12	1.4
100	861	99.7	3	11	826	96.8	27	3.2
200	864	100.0	0	0	858	99.3	6	0.7

¹N = 27 gDNA or wgaDNA samples were genotyped in duplicate using the AmpFlSTR^® Identifiler^® assay for N = 864 attempted genotypes/sample. ²A Genotype Quality Score (GQ) of <0.25 indicates a STR genotype with a Genotype Quality Score beloew the calling threshold of GeneMapper v3.0 software. ³gDNA exhibited significantly greater STR genotype completion rate than did 1 ng gDNA input (p < 0.001). ⁴gDNA exhibited significantly greater STR genotype concordance rates compared to wgaDNA (p < 0.0001, except for 50 ng gDNA input, with p = 0.001, and 200 ng gDNA input, p = 0.03).

ISSN: 1472-6750