gDNA Input (ng)
|
Completed1
|
% Completion
|
No Amplification
|
GQ<0.252
|
Concordant
|
% Concordance
|
Discordant Genotypes
|
% Discordance
|
---|
gDNA
|
864
|
100.03
|
0
|
13
|
851
|
100.04
|
0
|
0.0
|
1
|
821
|
95.0
|
43
|
70
|
638
|
80.1
|
150
|
19.0
|
10
|
862
|
99.8
|
2
|
9
|
830
|
97.1
|
25
|
2.9
|
25
|
863
|
99.9
|
1
|
22
|
823
|
97.7
|
19
|
2.3
|
50
|
863
|
99.9
|
1
|
15
|
836
|
98.6
|
12
|
1.4
|
100
|
861
|
99.7
|
3
|
11
|
826
|
96.8
|
27
|
3.2
|
200
|
864
|
100.0
|
0
|
0
|
858
|
99.3
|
6
|
0.7
|
- 1N = 27 gDNA or wgaDNA samples were genotyped in duplicate using the AmpFlSTR® Identifiler® assay for N = 864 attempted genotypes/sample. 2A Genotype Quality Score (GQ) of <0.25 indicates a STR genotype with a Genotype Quality Score beloew the calling threshold of GeneMapper v3.0 software. 3gDNA exhibited significantly greater STR genotype completion rate than did 1 ng gDNA input (p < 0.001). 4gDNA exhibited significantly greater STR genotype concordance rates compared to wgaDNA (p < 0.0001, except for 50 ng gDNA input, with p = 0.001, and 200 ng gDNA input, p = 0.03).