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Table 1 Immunoprecipitation capacity of 1 μl of human sera for Ruc-tumor antigen fusion proteinsa

From: A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renillaluciferase-antigen fusion proteins

   Ruc p53 K-Ras Smad4 β-CAT-Δ1 c-Myc
Controls 1 194 19,319 480 10,582 269 4,752
  2 9 9,830 1,064 3,575 835 2,913
  3 8 5,236 445 1,773 211 2,006
  4 38 3,187 477 1,919 530 1,831
  5 14 11,908 795 6,884 161 3,346
  6 31 5,390 823 1,724 235 2,050
  7 76 22,526 1,909 6,996 259 11,816
  8 29 15,338 943 8,043 445 3,475
  9 10 12,282 1,162 19,380 215 3,623
  10 9 11,130 1,109 4,429 501 5,060
+ 3 SDb   214 30,234 2,237 22,788 997 12,874
Head and Neck 11 0 10,904 508 2,721 196 2,193
  12 0 31,593 c 738 4,822 465 3,801
  13 0 12,367 840 1,868 673 4,407
  14 13 14,705 1,012 5,666 195 1,837
  15 33 31,733 c 1,189 5,264 552 4,107
  16 121 4,828 621 980 279 1,974
  17 0 8,517 1,160 8,396 336 2,958
  18 0 19,240 1,283 9,485 327 1,814
  19 0 11,224 1,517 4,454 410 4,370
  20 28 7,322 554 2,261 723 2,343
Breast 21 44 13,211 960 10,219 308 5,988
  22 10 18,814 696 42,970 c 302 5,450
  23 38 14,598 608 8,484 339 4,336
  24 77 11,587 1,655 17,297 2363 c 3,431
  25 17 19,954 532 10,184 772 15,650 c
  26 25 9,538 195 5,962 300 1,646
  27 10 7,815 2,561 c 20,628 426 3,524
  28 21 15,607 308 7,380 284 1,579
  29 0 18,058 160 6,790 304 2,333
  30 245 c 25,479 1,919 9,727 495 3,787
Colon 31 4 6,656 1,204 3,252 267 1,763
  32 40 20,928 4,293 c 5,567 962 6,143
  33 42 34,703 c 1,472 10,830 716 4,906
  34 51 300,943 c 6,439 c 2,610 992 3,789
  35 35 5,670 3,306 c 3,860 477 1,772
  36 44 6,516 695 37,344 c 371 2,395
  1. aSera, FLAG-Ruc-fusion extracts, protein A/G beads and buffer were mixed together, incubated for 60 minutes and processed. The data, light units, is the average of two experiments and is corrected for background (beads plus extract, but no sera). The standard deviation for each value is also available (see Additional file 1).
  2. bValues of the averages of the 10 control sera plus 3 standard deviations.
  3. cNumbers in bold are statistically significant: greater than the average plus 3 standard deviations of the 10 control sera.