Figure 3

Competition assays blocking Ruc-p53 immunoprecipitation using bacterially-produced antigen. Different amounts of E. coli-produced MBP-p53 were incubated with patient sera 34 (0.5 μl) or commercial anti-p53 antibody (25 ng) for 1 h. Protein A/G beads and Ruc-p53 extract were then added and incubated for an additional 1 h, processed and light units measured. The data shown is from one of two independent experiments giving similar results.