Figure 1From: A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renillaluciferase-antigen fusion proteinsImmunoprecipitation experiments with commercial antibodies. Various amounts of anti-FLAG monoclonal, anti-p53 polyclonal or control (anti-WASP) polyclonal antibodies were mixed with 5 μl of a Cos1 extract containing Ruc-p53 for 1 h in the presence of protein A/G beads, processed and light units measured. The data shown is from one of three independent experiments giving similar results.Back to article page