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Table 1 Influence of different genomic loci on de novo HAC formation

From: Efficient assembly of de novohuman artificial chromosomes from large genomic loci

Genomic Locus Size (kb) Location No. Transfections Freq. HAC formation1
G2 100 9q33 12 1/21 (5%)
G4 98 8q24.3 10 8/19 (42%)
G6 90 7p13 6 9/12 (75%)
G8 125 15q23 5 1/8 (12%)
G10 125 3p16 9 3/16 (19%)
G11 100 22q11.4 11 9/33 (27%)
G14 125 1q32 2 3/14 (21%)
G16 97 2p23 5 5/7 (71%)
G17 97 4q22 8 2/19 (10%)
G19 95 11p13 6 1/26 (4%)
ß-globin 216 11p15.5 6 7/27 (26%)
HGH 159 17q24.2 8 3/16 (19%)
PKD1 208 16p13.3 10 4/19 (21%)
  1. 1: HAC formation frequency is listed as (# clones containing HAC functionally validated by cytogenetic analysis and mitotic stability for at least 90 days)/(Total # clones screened from all transfections with the genomic locus of interest)