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Figure 4 | BMC Biotechnology

Figure 4

From: Efficient assembly of de novohuman artificial chromosomes from large genomic loci

Figure 4

Analysis of ß-globin mRNA expression from cell lines containing de novo ß-globin HACs after 30 days in the absence of selection. Poly A+ RNA from individual cell clones was subjected to first strand synthesis and PCR with primers specific to exon-3 of the ß-globin gene. Arrow indicates the ß-globin PCR product. 1) Untransfected HT1080. (2) Untransfected HT1080, no reverse transcriptase (RT). (3) ß-globin HAC clone #1, +RT. (4) ß-globin HAC clone #1, -RT. (5) ß-globin HAC clone #2, +RT. (6) ß-globin HAC clone #1, -RT. (7) ß-globin genomic DNA control

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