Figure 2
From: Efficient assembly of de novohuman artificial chromosomes from large genomic loci
Pulsed Field Gel Electrophoresis showing creation of HAC vector. Ligation reactions were set up using linearized BAC-CEN alone (Lane 1), linearized BAC-GEN alone (Lane 2), or linearized BAC-CEN and BAC-GEN together (Lane 3). A clear ligation product (arrow), the linear HAC vector, is only visible in Lane 3. Note that trace amounts of re-circularized CEN and GEN arms are detectable in Lanes 1 and 2, but these do not significantly affect assembly or purification of the HAC.