Figure 1From: Efficient assembly of de novohuman artificial chromosomes from large genomic lociStrategy for construction of a bimolecular, prefabricated, linear HAC vector. Digestion of BAC-CEN and BAC-GEN vectors with the ultra-rare homing endonucleases I-CeuI and PI-SceI permits directional ligation of both "arms" to form a linear HAC vector.Back to article page