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Figure 1 | BMC Biotechnology

Figure 1

From: Genetic and spectrally distinct in vivoimaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein

Figure 1

Co-visualization of multiple fluorescent proteins in mouse embryonic stem (ES) cells. Mixed colony of embryonic stem (ES) cells comprised of wild type (untagged) cells, CAG::ECFP transgenic cells exhibiting widespread expression of ECFP, CAG::EGFP transgenic cells exhibiting widespread expression of EGFP and CAG::mRFP1 transgenic cells exhibiting widespread expression of mRFP1. Bright field image (A), CFP channel (B), GFP channel (C), RFP channel (D), merge of all three fluorescent channels overlayed on the bright field image (E), merge of all three fluorescent channels (F), color-coded depth projection of the three fluorescent channel merge with the color-coded scale shown on the right of the image (G). A second mixed transgenic ES cell colony with bright field image (H), merge of three fluorescent channel merge overlayed on the bright field image (I) and dark field three fluorescent channel merge (J). In all panels except G ECFP fluorescence is shown in blue, EGFP fluorescence is in green and mRFP1 fluorescence is in red. bf, bright field; df, dark field.

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BMC Biotechnology

ISSN: 1472-6750

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