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Table 2 Correlation between hsp70i copy numbers in single blastomeres and whole 8-cell embryos, ± heat shock

From: Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell

 

hsp70i DNA copies

No-heat shock, No RT

Whole embryos (4)a

Per blastomere average ± s.d.

5 ± 2

 

hsp70i DNA + RNA copies

No-heat shock, + RT

Whole embryos (4)

Per blastomere average ± s.d.

19 ± 7

Heat shock, + RT

  
 

Embryo 1b

Embryo 2c

Blastomere 1

459

750

Blastomere 2

311

705

Blastomere 3

438

281

Blastomere 4

667

730

Blastomere 5

563

491

Blastomere 6

 

1251

Per blastomere average ± s.d.

488 ± 134

701 ± 325

Whole embryosb (6)

Per blastomere average ± s.d

500 ± 172

 

Whole embryosc (2)

Per blastomere average

 

650

  1. a The number of single embryos analyzed in each case is shown in parentheses.
  2. b Heat shock followed by 2-hour recovery prior to analysis.
  3. c Heat shock followed by 3-hour recovery prior to analysis.