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Table 1 Comparison of hsp70i copy numbers measured in single blastocysts: multistep/multitube versus PurAmp method

From: Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell

 

hsp70iDNA + RNA copies

 

Multistep/multitube extraction

PurAmp

No Heat shock, + RT

512 ± 69 (3)a

967 ± 548 (4)

Heat shock, + RT

2240 ± 1184 (3)

18,800 (2)

  1. a The number of single embryos averaged in each case is shown in parentheses.
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BMC Biotechnology

ISSN: 1472-6750

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