Figure 6

Easiness and versatility of the PurAmp method. In a streamlined version of the procedure, LysoDots (20 nl or less, see Methods for a definition) are dispensed to the lids of PCR tubes resting on a heating block. The LysoDots are quickly dried before adding the samples in a minimal volume of PBS. A second brief heating reduces the lysed samples to the semi-dry state, ready for storage or immediate processing, which may include a variable number and type of enzymatic reactions prior to the final PCR step. This will be a real-time PCR assay when quantification of the templates is desired.