Figure 5

Introduction of a DNase step within the single-tube PurAmp protocol for Xist/Sry quantification. Effect of DNase digestion on Xist (upper panel) and Sry (lower panel) template copy numbers measured in male and female single embryos at the morula stage. Male embryos: blue, non-treated; yellow, DNase-treated. Female embryos: red, non-treated; green, DNase-treated. The DNase enzyme displayed full activity under the conditions tested, as shown by the absence of genomic DNA in treated male samples and detailed in the text.