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Table 1 Promoters analysed in this study. The promoters (column 1 and 4) were amplified by PCR using the template DNA (column 2) and the primers (column 3) listed in the table.

From: Viral promoters can initiate expression of toxin genes introduced into Escherichia coli

Promoter fragment Template DNAa Primer sequenceb Size of promoter-fragment
P PH: Polyhedrin promoter from baculovirus pBacPAK8 [29] PH-1: GCCCGGGCCATCTCGCAAATAAATAAG
PH-2: GTCTAGACAGGGATCCGTATTTATAGG
78 bp
P CMV: Enhancer and immediate early promoter from CMV pRL-CMV [30] CMV-1: GCCCGGGGATCTTCAATATTGGCCATTAGCC
CMV-2: GTCTAGACACTGACTGCGTTAGCAATTTAAC
802 bp
P SV40: Early promoter from SV40 pGL3 [31] SV40-1: GCCCGGGCTAGCCCGGGCTCGAGATCTG
SV40-2: GTCTAGATTTGCAAAAGCCTAGGCCTCC
223 bp
P TK Thymidine kinase promoter from HSV1 pRL-TK [32] TK-1: GCCCGGGATCTAAATGAGTCTTCGGACCTCGC
TK-2: TCTAGACGAGACTGTTGTGTCAGAAGAATCAAGC
788 bp
P LTR HIV-1 subtype D 5'LTR promoter isolate 92UG021 LTR-1: GGCCCGGGATTAGATATCCACTGACCTTTGGATGGTGC
LTR-2: GGTCTAGATTAAGCAGTGGGTTCCCTAGCTAGCC
412 bp
P NPTIII: nptIII promoter pBin19 [33, 34] NPTIII-S: GCGCCCGGGCATAATTGTGGTTTCAAAATCGGC
NPTIII-AS: GGGTCTAGATTATTATTTCCTTCCTCTTTTC
193 bp
P BLA: TEM-1 promoter pKK232-8 [26, 35] Amp-S: GCGCCCGGGCGTCAGGTGGCACTTTTCG
Amp-AS: GGGTCTAGAACTCTTCCTTTTTCAATATTATTG
134 bp
  1. apBacPAK8 was from Clontech (Palo Alto, CA, USA), pRL-CMV, pGL3 and pRL-TK were from Promega (Madison, WI, USA). The 5'LTR promoter sequence was kindly provided by S. Somogyi.
  2. bRestriction sites added to the primers for cloning purposes are italicised.