Table 1 Promoters analysed in this study. The promoters (column 1 and 4) were amplified by PCR using the template DNA (column 2) and the primers (column 3) listed in the table.
From: Viral promoters can initiate expression of toxin genes introduced into Escherichia coli
Promoter fragment | Template DNAa | Primer sequenceb | Size of promoter-fragment |
|---|---|---|---|
P PH: Polyhedrin promoter from baculovirus | pBacPAK8 [29] | PH-1: GCCCGGGCCATCTCGCAAATAAATAAG PH-2: GTCTAGACAGGGATCCGTATTTATAGG | 78 bp |
P CMV: Enhancer and immediate early promoter from CMV | pRL-CMV [30] | CMV-1: GCCCGGGGATCTTCAATATTGGCCATTAGCC CMV-2: GTCTAGACACTGACTGCGTTAGCAATTTAAC | 802 bp |
P SV40: Early promoter from SV40 | pGL3 [31] | SV40-1: GCCCGGGCTAGCCCGGGCTCGAGATCTG SV40-2: GTCTAGATTTGCAAAAGCCTAGGCCTCC | 223 bp |
P TK Thymidine kinase promoter from HSV1 | pRL-TK [32] | TK-1: GCCCGGGATCTAAATGAGTCTTCGGACCTCGC TK-2: TCTAGACGAGACTGTTGTGTCAGAAGAATCAAGC | 788 bp |
P LTR HIV-1 subtype D 5'LTR promoter | isolate 92UG021 | LTR-1: GGCCCGGGATTAGATATCCACTGACCTTTGGATGGTGC LTR-2: GGTCTAGATTAAGCAGTGGGTTCCCTAGCTAGCC | 412 bp |
P NPTIII: nptIII promoter | pBin19 [33, 34] | NPTIII-S: GCGCCCGGGCATAATTGTGGTTTCAAAATCGGC NPTIII-AS: GGGTCTAGATTATTATTTCCTTCCTCTTTTC | 193 bp |
P BLA: TEM-1 promoter | pKK232-8 [26, 35] | Amp-S: GCGCCCGGGCGTCAGGTGGCACTTTTCG Amp-AS: GGGTCTAGAACTCTTCCTTTTTCAATATTATTG | 134 bp |