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Figure 4 | BMC Biotechnology

Figure 4

From: Cell membrane array fabrication and assay technology

Figure 4

Live cell assays for academic research and industrial drug discovery. (A) Control membranes (devoid of protein) or membranes reconstituted with either ICAM-1 or I-Ek-complexed to PCC peptide alone or together were incubated with murine T cells specific for PCC in the presence or absence of specific antibodies to either protein. Adhered cells were counted under the microscope and averaged from different corrals. (B) Murine T cells specific for PCC were incubated with supported lipid bilayers displaying fluorescently-labeled ICAM-1 and I-Ek – complexed to wild-type PCC, a mutated PCC*, or no peptide, and were subsequently assayed for the intensity of clustered I-Ek at the center of synapses. Values represent means ± SEM from at least 3 independent determinations.

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