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Figure 5 | BMC Biotechnology

Figure 5

From: CpG-island fragments from the HNRPA2B1/CBX3genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells

Figure 5

The 8.0 kb RNP fragment increases the yield of EPO in stably transfected CHO-K1 cells. A EPO production from pools of CHO-K1 cells stably transfected with hCMV-EPO or 8.0 kb RNP-EPO. Following selection on G418 pools of transfectants were cultured continuously with drug selection for 174 generations. Assays for EPO production were conducted every few days. B EPO production from clonal CHO-K1 cell lines derived with hCMV-EPO or 8.0 kb RNP-EPO. Clonal lines were derived by limiting dilution from pools of transfectants selected on G418. Colonies were expanded for 35 generations with drug selection prior to assay for EPO production.

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