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Figure 3 | BMC Biotechnology

Figure 3

From: CpG-island fragments from the HNRPA2B1/CBX3genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells

Figure 3

RNP CpG island fragments enhance the level and stability of EGFP expression in stably transfected CHO-K1 cells. A. Histograms representing the median fluorescence and percentage cells with detectable EGFP expression for CHO-K1 cells stably transfected with hCMV-EGFP, 8.0 kb RNP-EGFP or 4.0 kb RNP-EGFP. Following transfection pools of >100 transfectants were cultured continuously with drug selection for 107 generations and FACScan analysis conducted every few days. B. Histograms representing the median fluorescence and percentage cells with detectable EGFP expression for CHO-K1 cells stably transfected with hCMV-EGFP, 4.0 kb RNP-EGFP or 1.5 kb RNP-EGFP. Following transfection pools of >100 transfectants were cultured continuously with drug selection for 107 generations and FACScan analysis conducted every few days

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BMC Biotechnology

ISSN: 1472-6750

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